Modifying Enzymes
Thermo Scientific™ T4 Polynucleotide Kinase (10 U/μL)
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.
Thermo Scientific™ DNase I, RNase-free (supplied with MnCl2) (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Thermo Scientific™ RNase A/T1 Mix
Get the RNA degradation activity of both RNase A and RNase T1 with Thermo Scientific™ RNase A/T1 Mix.
Thermo Scientific™ Exonuclease I (20 U/μL)
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.
Invitrogen™ TrueCut™ HiFi Cas9 Protein (5 μg/μL)
A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.
Thermo Scientific™ RNase I (10 U/μL)
Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.
Invitrogen™ Ambion™ RNase III
Double-stranded RNA (dsRNA) specific endoribonuclease
Thermo Scientific™ Micrococcal Nuclease (300 U/μL)
Digest single-stranded and double-stranded DNA and RNA with Thermo Scientific™ Micrococcal Nuclease (S7 Nuclease), an endo-exonuclease.
Thermo Scientific™ Terminal Deoxynucleotidyl Transferase (20 U/μL)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the template-independent addition of deoxyribonucleotides to the 3'-OH terminus of DNA molecules.
Thermo Scientific™ Uracil-DNA Glycosylase (1 U/μL)
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.
Thermo Scientific™ T4 DNA Ligase, LC (1 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ Lambda Exonuclease (10 U/μL)
Lambda Exonuclease is a highly processive exodeoxyribonuclease that selectively digests the 5'-phosphorylated strand of double-stranded DNA in 5'-3' direction.
Corning™ Dispase
Bacillus-derived neutral metalloprotease that is recommended for recovering cells cultured on BD Matrigel™ Basement Membrane Matrix
Invitrogen™ LR Clonase™ II Plus enzyme
Mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor), and Xis (Excisionase) enzymes.
Invitrogen™ M13KO7 Helper Phage
Suitable for use with M13/phagemid hosts for the production of ssDNA from vectors containing an f1or f1-like origin of replication